m-mlv reverse transcriptase master mix (Promega)

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Promega m-mlv reverse transcriptase master mix
M Mlv Reverse Transcriptase Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/m-mlv+reverse+transcriptase+and+master+mix/m+mlv+reverse+transcriptase+and+master+mix/pm37783689-258-4-12
Average 90 stars, based on 1 article reviews

m-mlv reverse transcriptase master mix - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Soluble LR11/SorLA represses thermogenesis in adipose tissue and correlates with BMI in humans
Article Snippet: Complimentary DNA was generated from 500 ng of RNA using M-MLV reverse transcriptase and master mix (Promega) in a 20 μl reaction mixture with 2.5 mM MgCl 2 , 1.25 mM dNTPs and 5 μg ml −1 random hexamers at 37 °C for 1 h. Complementary DNA was diluted 75-fold and 5 μl of diluted complementary DNA was used in a 12 μl real-time PCR reaction mixture using TaqMan primers and probes or SYBR green reagent (Applied Biosystems) according to manufacturer's instructions.

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions
Article Snippet: Complimentary DNA was generated from 500 ng of RNA using M-MLV reverse transcriptase and master mix (Promega) in a 20 μl reaction with 2.5 mM MgCl 2 , 1.25 mM dNTPs and 5 μg/ml random hexamers at 37°C for 1 hr. cDNA was diluted 75 fold and 5 μl of diluted cDNA was used in a 12 μl real time PCR reaction using TaqMan primers and probes or SYBR green reagent (Applied Biosystems) according to manufacturer's instructions.

Article Title: Early peroxisome proliferator-activated receptor gamma regulated genes involved in expansion of pancreatic beta cell mass
Article Snippet: Complimentary DNA was generated from 500 ng of RNA using M-MLV reverse transcriptase and master mix (Promega) in a 20 μl reaction with 2.5 mM MgCl2, 1.25 mM dNTPs and 5 μg/ml random hexamers at 37°C for 1 hour. cDNA was diluted 75 fold and 5 μl of diluted cDNA was used in a 12 μl real time PCR reaction using TaqMan primers and probes or SYBR green reagent (Applied Biosystems) according to manufacturers instructions.

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions
Article Snippet: Complimentary DNA was generated from 500 ng of RNA using M-MLV reverse transcriptase and master mix (Promega) in a 20 ml reaction with 2.5 mM MgCl2, 1.25 mM dNTPs and 5 mg/ml random hexamers at 37 C for 1 hr. cDNA was diluted 75 fold and 5 ml of diluted cDNA was used in a 12 ml real time PCR reaction using TaqMan primers and probes or SYBR green reagent (Applied Biosystems) according to manufacturer’s instructions.

Article Title: Lipocalin Prostaglandin D Synthase and PPARγ2 Coordinate to Regulate Carbohydrate and Lipid Metabolism In Vivo
Article Snippet: Complimentary DNA was generated from 500 ng of RNA using M-MLV reverse transcriptase and master mix (Promega) in a 20 μl reaction with 2.5 mM MgCl 2 , 1.25 mM dNTPs and 5 μg/ml random hexamers at 37°C for 1 hour. cDNA was diluted 75 fold and 5 μl of diluted cDNA was used in a 12 μl real time PCR (RT-PCR) reaction using TaqMan primers and probes or SYBR green reagent (Applied Biosystems) according to manufacturers instructions.

Reverse Transcription:

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions
Article Snippet: Complimentary DNA was generated from 500 ng of RNA using M-MLV reverse transcriptase and master mix (Promega) in a 20 ml reaction with 2.5 mM MgCl2, 1.25 mM dNTPs and 5 mg/ml random hexamers at 37 C for 1 hr. cDNA was diluted 75 fold and 5 ml of diluted cDNA was used in a 12 ml real time PCR reaction using TaqMan primers and probes or SYBR green reagent (Applied Biosystems) according to manufacturer’s instructions.

Real-time Polymerase Chain Reaction:

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions
Article Snippet: Complimentary DNA was generated from 500 ng of RNA using M-MLV reverse transcriptase and master mix (Promega) in a 20 ml reaction with 2.5 mM MgCl2, 1.25 mM dNTPs and 5 mg/ml random hexamers at 37 C for 1 hr. cDNA was diluted 75 fold and 5 ml of diluted cDNA was used in a 12 ml real time PCR reaction using TaqMan primers and probes or SYBR green reagent (Applied Biosystems) according to manufacturer’s instructions.

SYBR Green Assay:

Article Title: BMP8B Increases Brown Adipose Tissue Thermogenesis through Both Central and Peripheral Actions
Article Snippet: Complimentary DNA was generated from 500 ng of RNA using M-MLV reverse transcriptase and master mix (Promega) in a 20 ml reaction with 2.5 mM MgCl2, 1.25 mM dNTPs and 5 mg/ml random hexamers at 37 C for 1 hr. cDNA was diluted 75 fold and 5 ml of diluted cDNA was used in a 12 ml real time PCR reaction using TaqMan primers and probes or SYBR green reagent (Applied Biosystems) according to manufacturer’s instructions.

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